Biotin NovaTag® Resin is a specialized solid-phase support designed for the efficient synthesis of C-terminally biotinylated peptides using Fmoc-based solid-phase peptide synthesis (SPPS). Manufactured by Novabiochem®, this resin integrates the biotin moiety directly into the linker, streamlining the biotinylation process and enhancing the quality of the final peptide product.sigmaaldrich-jp.comsigmaaldrich-jp.com+3MilliporeSigma+3MilliporeSigma+3
Key Features
Integrated Biotin Linker: The biotin is incorporated into the resin’s linker, eliminating the need for post-synthesis biotinylation steps.Scientific Labs
Improved Solubility and Reactivity: By embedding biotin within the linker, the resin addresses common issues associated with biotin’s poor solubility and sluggish coupling kinetics, leading to more efficient peptide synthesis .MilliporeSigma+1MilliporeSigma+1
Fmoc Compatibility: Fully compatible with standard Fmoc SPPS protocols, allowing for seamless integration into existing workflows.sigmaaldrich-jp.com+2MilliporeSigma+2MilliporeSigma+2
Efficient Cleavage: Peptide cleavage using trifluoroacetic acid (TFA) yields a biotinylated peptide without additional modifications.
Technical Specifications
Resin Matrix: Polystyrene cross-linked with 1% divinylbenzene (DVB)
Mesh Size: 100–200 mesh
Loading Capacity: Approximately 0.35–0.65 mmol/g (determined photometrically via Fmoc chromophore release) .MilliporeSigma
Appearance: White to light yellow to beige beadsMilliporeSigma
Swelling Volume: Lot-specific; optimized for solvents like DMF
Storage Conditions: Store at 2–30 °C in a dry environment
Applications
Biotin NovaTag® Resin is particularly suited for:Scientific Labs+5MilliporeSigma+5ChemicalBook+5
Synthesis of Biotinylated Peptides: Ideal for generating peptides with a biotin moiety at the C-terminus, commonly used in affinity purification, labeling, and detection assays.
High-Throughput Peptide Production: Facilitates the rapid synthesis of biotinylated peptides without the need for post-synthetic modifications.
Research in Molecular Biology and Biochemistry: Supports studies involving protein-protein interactions, enzyme assays, and cell-surface receptor investigations.
Availability & Pricing
Biotin NovaTag® Resin is available in various quantities. For instance, a 500 mg package (Product No. 855051) is priced at approximately $354.00 USD, while a 1 g package is around $651.00 USD . Prices may vary based on supplier and region. For the most accurate and up-to-date pricing, it’s recommended to consult authorized distributors or the manufacturer’s website.MilliporeSigma+4sigmaaldrich-jp.com+4ChemicalBook+4
Documentation & Safety
Safety Classification: Not classified as hazardous under Regulation (EC) No 1272/2008 .MSDS Manager
Safety Data Sheet (SDS): Available through Merck’s official websitesigmaaldrich-jp.com
For researchers and professionals engaged in peptide synthesis, Biotin NovaTag® Resin offers a reliable and efficient medium, ensuring high-quality results in both academic and industrial settings.
If you need further information on protocols or application methods, feel free to ask!
NOVATAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
Phone : +49 1512 4714765 ( Germany )
Phone : +1 (702) 381-3042 ( USA )
Email : [email protected]
Address : Cunostraße 56, 14193 Berlin. Germany
Address : 7373 Rowlett Park Dr, Tampa, FL 33610, United States.